Journal: Molecules

Article Title: Aberrant Expression of Bacterial Pattern Recognition Receptor NOD2 of Basophils and Microbicidal Peptides in Atopic Dermatitis

doi: 10.3390/molecules21040471

Figure Lengend Snippet: Expression of TLR2 and NOD2 in CD203-positive granulocytes. ( a ) Representative histogram is shown for the CD203+ cells gated from total granulocytes; ( b ) Representative histograms of the cell surface expression of TLR2 and intracellular expression of NOD2 of CD203c+ granulocytes are shown. ( c ) Box and whiskers plots showing the comparison of cell surface TLR2 and intracellular NOD2 expression (MFI) in CD203+granulocytes between AD patients and control subjects were determined by Mann-Whitney rank sum test. AD: patients with atopic dermatitis; Control: normal control subjects.

Article Snippet: Unconjugated mouse anti-TLR2 (Imgenex Corp., San Diego, CA, USA), anti-NOD2 antibody (Bio-legend Corp, San Diego, CA, USA), corresponding mouse IgG1, κ isotypic control antibody (BD Pharmingen Corp., San Diego, CA, USA), together with a fluorescein iso-thiocyanate (FITC)-conjugated goat anti-mouse IgG (H + L) secondary antibodies (Zymed Laboratories, Inc., South San Francisco, CA, USA), were used for staining.

Techniques: Expressing, Comparison, MANN-WHITNEY

Journal: Mediators of Inflammation

Article Title: Cholesterol Oxidase Binds TLR2 and Modulates Functional Responses of Human Macrophages

doi: 10.1155/2014/498395

Figure Lengend Snippet: The effect of ChoD on TLR-2 and CR3 expression on THP-1-derived macrophages. TLR2 (a) and CR3 (b) expression on cells treated with ChoD (0.01–0.5 U/mL) for 24 h. Data are means ± SEM of MFI values from 5-6 separate experiments (* P < 0.05 for macrophages + ChoD versus macrophages). (c) LTA (100 μ g/mL, 30 min) binding to macrophages pretreated with ChoD (0.5 U/mL) for 24 h. After treatment with ChoD and LTA, macrophages were incubated with anti-LTA antibodies and secondary FITC-conjugated antibodies and then analyzed using flow cytometry. Data are expressed as means ± SEM of MFI values from seven independent experiments (* P < 0.05 for macrophages + ChoD + LTA versus macrophages + LTA). Percentage values relative to control (100%) are shown on the right axis.

Article Snippet: Phycoerythrin (PE)-conjugated mouse IgG2a ( γ ) anti-human TLR2 antibodies (clone TL2.1) and PE-conjugated mouse IgG2a ( γ ) isotype control were from Imgenex (San Diego, CA, USA).

Techniques: Expressing, Derivative Assay, Binding Assay, Incubation, Flow Cytometry, Control